I used this antibody to stain murine 50um thick brain slices ( 4% PFA fixed brains). Prior to immunohistochemistry, the tissue were target retrieved using DAKO antigen retrieval s1699 according to protocol from the manufacturer.
This antibody works excellent for detection of aggregated mutant Huntingtin in mice suffering from Huntington’s. Nuclear aggregations were clearly detected using this antibody with very low background signal.
On May 31, 2019 Anders Dalby wrote:
I used this antibody to stain murine 50um thick brain slices ( 4% PFA fixed brains). Prior to immunohistochemistry, the tissue were target retrieved using DAKO antigen retrieval s1699 according to protocol from the manufacturer.
This antibody works excellent for detection of aggregated mutant Huntingtin in mice suffering from Huntington’s. Nuclear aggregations were clearly detected using this antibody with very low background signal.
Dilution 1:50 (overnight incubation)