GFAP, guinea pig pAb, Synaptic Systems, 173 004

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2 User Reviews

  • We use this antibody for IHC staining of mouse brain tissue.
    It works well for both un-perfused (cryosections) and 4% PFA perfused (vibratome sections) tissues.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • By applying this primary antibody to immunofluorescence labeling you might expect very good results in paraformaldehyde-fixed tissues

    Guinea pig-anti-glial fibrillary acidic protein (GFAP) from Synaptic Systems (Göttingen, Germany; 173004) – well usable combined with mouse, rabbit and goat antibodies. Importantly, the secondary antibodies directed against rabbit and guinea pig IgG should be not cross-reacting (available highly purified donkey antibodies are for instance from Jackson ImmunoResearch, West Grove, PA, USA; German supplier: Dianova, Hamburg). Some references are Antonucci, Alpár et al 2012, Fig 6;Härtig et al 2014 Neuropathol Appl Neurobiol, Fig 6; Michalski et al 2017 PloS One , Figs 2,3,10-13).

    Antonucci et al. 2012: http://www.jneurosci.org/content/32/6/1989.long
    Härtig et al. 2014: https://onlinelibrary.wiley.com/doi/full/10.1111/nan.12050
    Michalski et al. 2017: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174996

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM