Related topics: Alphabetical list, Apoptosis, Cancer biology, Cell cycle, Metabolism, Signal transduction, Transcriptional regulation
Genis et al. F1000Research 2014 used this antibody to monitor IGF-I induced Akt signaling in astrocytes by Western blotting. Dilution 1:1000.
See list of additional Phospho-Akt antibodies at Linscott’s Directory
On May 5, 2020
Margie Sutton, University of Texas MD Anderson Cancer Center wrote:
WB dilution 1:2000
IF rating: 5 stars
IHC rating: 4 stars
For more information see Sutton et al. Cell Reports 2019, “DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters”
On September 12, 2017
Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University wrote:
This antibody was used for immunoblotting of RIBA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
On January 23, 2017
Christina Demuth wrote:
This antibody is used for evaluating activity of the EGF-receptor pathway after stimulation or inhibition of EGFR in lung cancer cell lines. The antibody is very reliable. We have found that dilution in 5 % BSA is optimal.
On November 4, 2015
Anne Mayntzhusen (TVS group) wrote:
Phospho-Akt (Ser473) rabbit antibody #9271 from Cell Signaling gives one specific band at 60 kDa.
I have used the antibody for western blotting with 20 ug WCE of cultured primary mouse hippocampal neurons and with 20 ug WCE of the human neuroblastoma cell line SH-SY5Y in the dilution 1:1000 and incubation at +4C ON.
The antbody can be reused 2 times.
On March 20, 2014
Maria Lume wrote:
rabbit Phospho-Akt (cat no 9271) from Cell Signaling works very nicely in WB. Have detected pAKT levels from both cell-lines (MG87 fibroblasts) as well as from embryonic kidney tissue lysates (E14).
Used dilution 1:1000, 5%BSA/TBS/Tween, incubates O/N at +4C