CD8 (human), mouse mAb (clone RPA-T8), APC conjugated, BD Biosciences, 555369

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2 User Reviews

  • Recommended 20 ul per million cells. Used 10ul for 300,000 cells (PBMCs).
    Strong signal, optimal for staining of highly expressed extracellular markers.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometry44444
    LuminexNot Rated
    EMNot Rated
  • Used to perform single stainings of GFP positive CAR T cells to determine basic phenotype (CD3, CD4, CD8) before and after sorting using CD4/CD8 beads or flow cytometry cell sorting. Cells were stained in FACS tubes. No antibody titration or compensation were performed.
    Working solution of 20uL in 200uL sample to stain approx. 500.000 cells.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometry44444
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM