TNF (human), mouse mAb (clone MAb11), APC conjugated, BD Biosciences 551384

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2 User Reviews

  • Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.

    a total of 1,0uL used in a 20uL mastermix with anti-IFN used to stain approx. 300.000 cells.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometry44444
    LuminexNot Rated
    EMNot Rated
  • Used to stain cells for the intracellular expression of cytokines in an intracellular cytokine secretion (ICS) assay (TNF, IFNy, CD107a). Antibody mixed in mastermix to stain cells in FACS tubes after assay incubation and after permeabilization. Antibody titrations and compensation were previously performed in-lab.
    Working solution of 1uL in 20uL mastermix to stain approx. 300.000 cells.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometry44444
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM