α-adrenergic receptor (AR) subtypes α2C-AR, mouse mAb clone N330A/80, Abcam, ab167433

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  • In “Commercially available antibodies directed against α-adrenergic receptor subtypes and other G protein-coupled receptors with acceptable selectivity in flow cytometry experiments”, Tripathi et al study the specificity of 8 different antibodies against α-adrenergic receptor (AR) subtypes. The specificity of the antibodies was tested by flow cytometry using human vascular smooth muscle cell. Here, a fluorescent signal was measured when treated with siRNA specific for the α-adrenergic receptor (Human ADRA2C (152) (E-005424-00-0050)) and compared with the fluorescent signal from cells treated with non-targeting RNA.

    When using this antibody, a 70% reduction in fluorescence was observed.

    See full description in the article:
    https://www.ncbi.nlm.nih.gov/pubmed/26660071

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM