Shb, rabbit pAb, custommade by Genscript

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One User Review

  • This antibody was evaluated in a study of commercially available antibodies directed against SH2 domain-containing adapter protein B (Shb). Nine antibodies were included in the study and their performance was tested in Western blotting and Immunoprecipitation.

    Tests were performed using cell lysate obtained from the cell line HEK 293T, which endogenously expresses Shb, and HEK 293T cells transiently transfected with CMV promoter driven overexpression of Shb. As a negative control, Shb was knocked-down in HEK 293T-derived cell line CE12 using Shb-specific siRNA.

    In WB, this antibody detected overexpressed Shb, thus it recognizes Shb but only very weakly.

    The antibody was not tested in IP.

    Immunogen: The whole recombinant human Shb protein
    Lot number: Custom made
    Dilution: 1 µg/ml

    Results are summarized in inserted table.

    For further information see: Evaluation and validation of commercial antibodies for the detection of Shb. Vanli, Cuesta-Marban and Widmann, PLOS ONE December 1, 2017.

    Western blot22222
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Flow cytometry