Gephyrin, mouse mAb, clone mAb7a, Synaptic Systems, 147 021

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2 User Reviews

  • This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
    Dilution used: 1:2000

    For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).

    Hughes Laboratory:
    https://specciebear.wixsite.com/spinal-cord-hughes

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Gephyrin, mouse mAb, mAb7a hybridoma supernatant, Synaptic Systems 147 021,

    ICC (1:500-1:1000)

    Great antibody, stains specificly inhibitory postsynaptic densities in primary hippocampal neruons. I didn’t observe any unspecific staining of the nucleus for the hybridoma supernatant as opposed to the mAb7a purified IgG (mentioned in my review of 147 108).

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM