Anti-human IFN-gamma mAb, clone: 1-D1K, MABTECH, #3420-3
mAb used as coating antibody in ELISpot assay to detect cellular immune response of chimeric antigen receptor modified T-cells against CD19 positive B-cells.
Anti-hIFNy was diluted in PBS to a final concentration of [7.5 ug/mL] (coating volume: 75uL) (incubation ON at RT)
Used in combination with biotinylated anti-human IFNy detection antibody (clone: 7-b6-1).
Plate was washed between each step using 6 x 200uL PBS (or PBS + 0.05% tween).
Very good results were obtained with clear individual spots, a clear dose-dependent response and low background. Positive control: staphylococcal enterotoxin B [2ug/mL].
On June 29, 2017 Kasper Mølgaard Jensen wrote:
Anti-human IFN-gamma mAb, clone: 1-D1K, MABTECH, #3420-3
mAb used as coating antibody in ELISpot assay to detect cellular immune response of chimeric antigen receptor modified T-cells against CD19 positive B-cells.
Anti-hIFNy was diluted in PBS to a final concentration of [7.5 ug/mL] (coating volume: 75uL) (incubation ON at RT)
Used in combination with biotinylated anti-human IFNy detection antibody (clone: 7-b6-1).
Plate was washed between each step using 6 x 200uL PBS (or PBS + 0.05% tween).
Very good results were obtained with clear individual spots, a clear dose-dependent response and low background. Positive control: staphylococcal enterotoxin B [2ug/mL].