Histone H4K20me3, rabbit pAb, Millipore, 07-463

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One User Review

  • Histone H4K20me3, rabbit, Millipore #07-463

    Tested on dilution of histone extract (acid extraction, mouse brain) and cell lysates (RIPA buffer, mouse brain). Seems to have rather low affinity for histones in the cell extract (many aspecific bands, extremely weak band op appropriate MW). Histone extraction recommended.

    WB details: BIS-TRIS gel+XT MES running buffer, blocked in TBS+5%BSA, 1:2000 dilution (picture inserted)

    Western blot44444
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM