VGLUT2, rabbit pAb, Synaptic Systems, 135 403

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4 User Reviews

  • I highly recommend this antibody.

    Affinity-purified rabbit antibodies directed against the vesicular glutamate transporters (VGLUT2; Synaptic Systems, 135403) were repeatedly found to be useful for a subpopulation of glutamatergic endings for instance in mice (Michalski et al 2013, Figs 4 and 6) and hamsters (Härtig et al 2007, Fig 7) These antibodies might be also concomitantly applied with affinity-purified antibodies recognizing VGLUT1 (also available from Synaptic Systems) to simultaneously reveal the vast majority of glutamatergic endings.

    Michalski et al. 2013: https://www.ncbi.nlm.nih.gov/pubmed/23219666
    Härtig et al. 2007: https://onlinelibrary.wiley.com/doi/full/10.1111/j.1460-9568.2006.05250.x

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I performed IHC with this antibody on 12 um mouse olfactory bulb cryosections to stain glomerular layer. Specific staining in axon terminals but there is background.
    Antibody dilution:1/500

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Works for Western blotting using membrane preparations of mouse hippocampus.
    Dilution 1:500

    Western blot33333
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Useful antibody as a presynaptic marker in subsets of primary mouse hippocampal neurons.

    IF dilution 1:100

    Western blotNot Rated
    Immunostaining44444
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
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Luminex