The antibody was used for Immunofluorescence staining of neurons derived from murine ES cells differentiated in culture to investigate the presents of dopaminergic neurons.
The cells were grown on coated cover slips, fixed for 30min in 4% PFA and treated for 5 min with 0,005% Triton-X-100. To reduce unspecific binding cells are blocked/ quenched with a 1% BSA in PBS solution.
The Antibody was diluted 1:500. The secondary antibody was a goat anti mouse 488 diluted 1:1000.
A clear and strong staining of the cytoplasm into the neurites was observed. The antibody worked well in double staining experiments with Sigma # A2052 Rabbit-anti-GABA (1:250).
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On April 16, 2012Simon Glerup, Aarhus wrote:
4.5
Excellent antibody for Western blotting.
We have used it on a number of mouse tissues.
It also works for IF staining of primary mouse dopaminergic neurons.
Also works for immunohistochemistry on mouse brain tissues following blocking with anti-mouse Fab fragments. Some background staining still remains following blocking.
On May 31, 2016 Urs Kindler wrote:
The antibody was used for Immunofluorescence staining of neurons derived from murine ES cells differentiated in culture to investigate the presents of dopaminergic neurons.
The cells were grown on coated cover slips, fixed for 30min in 4% PFA and treated for 5 min with 0,005% Triton-X-100. To reduce unspecific binding cells are blocked/ quenched with a 1% BSA in PBS solution.
The Antibody was diluted 1:500. The secondary antibody was a goat anti mouse 488 diluted 1:1000.
A clear and strong staining of the cytoplasm into the neurites was observed. The antibody worked well in double staining experiments with Sigma # A2052 Rabbit-anti-GABA (1:250).
On April 16, 2012 Simon Glerup, Aarhus wrote:
Excellent antibody for Western blotting.
We have used it on a number of mouse tissues.
It also works for IF staining of primary mouse dopaminergic neurons.
Also works for immunohistochemistry on mouse brain tissues following blocking with anti-mouse Fab fragments. Some background staining still remains following blocking.
IF and IHC 1:1000
WB 1:2000