ZO-1 (zonula occludens 1), rabbit pAb, Thermo Fisher Scientific, 61-7300

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  • ZO-1 (zonula occludens 1)
    Thermo, 61-7300

    We used this antibody for IHC-labeling of human breast cancer tissue. The antibody gave a specific labeling of classical tight juntion “punctae” in ducts.

    The antibody was used at 1:400 ON. The tissue was PFA-fixed, embedded in paraffin, cut at 3 um, and target retrival was performed in TEG-buffer (Tris/EGTA). The secondary antibody was used at 1:200 and developed with DAB for 1 min 15 sec.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM