8 User Reviews

  • Excellent for staining pfa fixed primary hippocampal neurons.

    Neurons from p0 pups were grown for 10 days in vitro before being fixed. Following fixation, the neurons were immunostained against sortilin (1:100). A clear and intense signal was observed with almost no background. Highly recommended!

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody works very well for WB, IP and ELISA. It also works for indirect IF staining to see endogenous Sortilin in primary macrophages and dendritic cells.

    Western blot55555
    Immunostaining55555
    IP55555
    ELISA55555
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody works very well for immunofluorescence staining in rat astrocytes and brain endothelial cells. Dilution 1:200. Fixation was done both with paraformaldehyde and methanol.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody works fine to detect endogenous levels of sortilin in mouse dorsal root ganglia and sciatic nerve homogenates (50 ug total protein), but it is not very sensitive for detection of sortilin in primary rat Schwann cell lysates (100 ug total protein) . Dilution 1:1000.

    Western blot33333
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • The antibody was used for double immunofluorescence stainings on cryo-sections of mouse brain, and with a concentration of 0.22 ug/mL (1:9000) it is still possible to get a nice staining with low background.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody is excellent for IHC on dorsal root ganglia in mice.

    Dilution: 1:100

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody works very well for IHC of mouse spinal cord cryo-sections and dorsal root ganglia. I have also used it for ICC of primary dorsal root ganglia neurons. The immunofluorescence staining is vesicular and looks very specific. Dilution: 2 ug/mL.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody is great for immunohistochemistry and immunocytochemistry on mouse cryosections and primary neurons, respectively. Dilution 2 ug/ml. See image above for sortilin (green) in a DIV12 hippocampal neuron.

    It is also in our hands the best antibody for immunoprecipitation of endogenous mouse sortilin.

    Western blotNot Rated
    Immunostaining55555
    IP55555
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
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ELISA
Flow cytometry
Luminex