Related topics: Signal transduction
See list of additional p-RSK antibodies at Linscott’s Directory
On November 28, 2017 Anders Dalby wrote:
This antibody works very well with clear bands and almost no background signal.
Dilution: 1:1000
On January 31, 2017 Simon Mølgaard Jensen, Department of Biomedicine, Aarhus University wrote:
p-RSK ab32413 (formerly know as MBL int. polyclonal antibody, AT-7163)
I have tested this on lysates from mouse hippocampal neurons with great results!
The neurons were stimulated with 1nM BDNF for 10 minutes before lysis in lysis-buffer containing phos-stop and complete.
50ug protein was loaded per sample. The antibody was used at a 1:1000 dilution and left to incubate over night at 4 degrees.
A clear signal was observed in the sample stimulated with BDNF while a very weak band was observed in the unstimulated sample.
Δ
On November 28, 2017 Anders Dalby wrote:
This antibody works very well with clear bands and almost no background signal.
Dilution: 1:1000
On January 31, 2017 Simon Mølgaard Jensen, Department of Biomedicine, Aarhus University wrote:
p-RSK ab32413 (formerly know as MBL int. polyclonal antibody, AT-7163)
I have tested this on lysates from mouse hippocampal neurons with great results!
The neurons were stimulated with 1nM BDNF for 10 minutes before lysis in lysis-buffer containing phos-stop and complete.
50ug protein was loaded per sample. The antibody was used at a 1:1000 dilution and left to incubate over night at 4 degrees.
A clear signal was observed in the sample stimulated with BDNF while a very weak band was observed in the unstimulated sample.