This antibody was used for immunoblotting of RIBA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
Dilution: 1:1000
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 30, 2017Christina Demuth, Department of Clinical Medicine, Aarhus University wrote:
5
Phospho-Src (Tyr416), Cell signaling 2101 (rabbit)
The antibody was used on sarcoma cell lines after treatment with the Src-inhibitor dasatinib , to evaluate the effect. For this purpose the antibody was great. The manufacturer recommends diluting in 5 % BSA, that worked for us, though we increased the antibody concentration.
Dilution: 1:500
On September 12, 2017 Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University wrote:
This antibody was used for immunoblotting of RIBA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
Dilution: 1:1000
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
On January 30, 2017 Christina Demuth, Department of Clinical Medicine, Aarhus University wrote:
Phospho-Src (Tyr416), Cell signaling 2101 (rabbit)
The antibody was used on sarcoma cell lines after treatment with the Src-inhibitor dasatinib , to evaluate the effect. For this purpose the antibody was great. The manufacturer recommends diluting in 5 % BSA, that worked for us, though we increased the antibody concentration.
Dilution: 1:500