On January 30, 2017lars meinertz byg, Department of Biomedicine, Aarhus University wrote:
3
Phospho-eIF4G (Ser1108) Rabbit-Antibody #2441 CST
I used this antibody on stimulated and unstimulated murine hippocampal neurons. There may be a specificity for phosphorylated eIF4G as a band is seen in the stimulated lane around the 191 kDa-marker, however there is a stronger band with weaker respons to stimulation right around 240 kDa. hard to know which one to quantitate. (see picture)
Konc.: 1:1000
exposure-time: 30 min
lane-load: 50ug
Please see inserted image
On January 30, 2017 lars meinertz byg, Department of Biomedicine, Aarhus University wrote:
Phospho-eIF4G (Ser1108) Rabbit-Antibody #2441 CST
I used this antibody on stimulated and unstimulated murine hippocampal neurons. There may be a specificity for phosphorylated eIF4G as a band is seen in the stimulated lane around the 191 kDa-marker, however there is a stronger band with weaker respons to stimulation right around 240 kDa. hard to know which one to quantitate. (see picture)
Konc.: 1:1000
exposure-time: 30 min
lane-load: 50ug
Please see inserted image