I have tested this antibody on mouse HepG2 cells.
50ug protein were loaded in each well for western blot.
Dilution: 1:1000
The membrane was left to incubate over night at 4 C in blocking buffer (milk).
The antibody did not work.
This antibody works well on mouse liver lysates to detect total CREB however does not work on primary murine neuronal lysates. Dilution 1:1000.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On January 8, 2017Signe Petersen, Aarhus University wrote:
1
CREB (48H2), Rabbit mAb #9197, Cell Signaling Technologies
I have used this antibody for detection of total CREB without any valuable results.
Western blotting of murine hippocampal neurons in a dilution of 1:1000, as recommended by the supplier, did not yield any results. Reprobing the same blot with beta-actin proved that the blotting procedure was succesful.
On October 3, 2018 Michelle Pedersen wrote:
I have tested this antibody on mouse HepG2 cells.
50ug protein were loaded in each well for western blot.
Dilution: 1:1000
The membrane was left to incubate over night at 4 C in blocking buffer (milk).
The antibody did not work.
On September 21, 2018 Anders Dalby wrote:
This antibody works well on mouse liver lysates to detect total CREB however does not work on primary murine neuronal lysates. Dilution 1:1000.
On January 8, 2017 Signe Petersen, Aarhus University wrote:
CREB (48H2), Rabbit mAb #9197, Cell Signaling Technologies
I have used this antibody for detection of total CREB without any valuable results.
Western blotting of murine hippocampal neurons in a dilution of 1:1000, as recommended by the supplier, did not yield any results. Reprobing the same blot with beta-actin proved that the blotting procedure was succesful.