great for IP of Murine SorCS2 in a 1ug/IP solution.
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 30, 2015Lars Meinertz Byg wrote:
5
Good for IP of SorCS2. Has higher affinity for the 2-chain receptor.
I Used it on stable hSorCS2-transfected SY5Y-cells in a concentration of 1ug/IP
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 15, 2015Gangadaar Thotakura wrote:
3
Used for Hek293T cell line transfected with mutated Sorcs2 variants. I used for western blot and immunofluorescence. Western blot didn’t work but it works excellently for immunofluorescence.
Dilution for western blot: 10ug/ml
Dilution for immunofluorescence: 10ug/ml
Western blot
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 18, 2013Ann-Kathrin Reuschlein wrote:
5
Used in RN22 cells transfected with human SorCS2 variants, cross-linked with DSP.
2 ug per IP.
Gives a good IP better than10 %.
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 18, 2013Ann-Kathrin Reuschlein wrote:
0
Used in RN22 cells transfected with human SorCS2 variants, cross-linked with DSP.
2 ug per IP. Gives a good IP better than10 %.
On March 2, 2012Simon Glerup, Aarhus wrote:
3
Mouse monoclonal antibody generated in the lab of Anders Nykjær, Aarhus University.
Works in immunofluorescense using PFA-fixed primary mouse hippocampal neurons. 10 ug/ml
Knockout neurons were used as negative control.
On February 25, 2016 Lars Meinertz Byg wrote:
great for IP of Murine SorCS2 in a 1ug/IP solution.
On November 30, 2015 Lars Meinertz Byg wrote:
Good for IP of SorCS2. Has higher affinity for the 2-chain receptor.
I Used it on stable hSorCS2-transfected SY5Y-cells in a concentration of 1ug/IP
On January 15, 2015 Gangadaar Thotakura wrote:
Used for Hek293T cell line transfected with mutated Sorcs2 variants. I used for western blot and immunofluorescence. Western blot didn’t work but it works excellently for immunofluorescence.
Dilution for western blot: 10ug/ml
Dilution for immunofluorescence: 10ug/ml
On November 18, 2013 Ann-Kathrin Reuschlein wrote:
Used in RN22 cells transfected with human SorCS2 variants, cross-linked with DSP.
2 ug per IP.
Gives a good IP better than10 %.
On November 18, 2013 Ann-Kathrin Reuschlein wrote:
Used in RN22 cells transfected with human SorCS2 variants, cross-linked with DSP.
2 ug per IP. Gives a good IP better than10 %.
On March 2, 2012 Simon Glerup, Aarhus wrote:
Mouse monoclonal antibody generated in the lab of Anders Nykjær, Aarhus University.
Works in immunofluorescense using PFA-fixed primary mouse hippocampal neurons. 10 ug/ml
Knockout neurons were used as negative control.