HLA Class I, mouse mAb clone W6/32, Sigma-Aldrich, H1650

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2 User Reviews

  • anti-HLA CLass I mAb, clone W6/32
    Sigma

    Used at 5 uL/million cells, detection with GAM-PE

    This antibody is used at my lab to detect human MHC class I, as a positive control (especially on cell lines which are being used as negative controls for the experiment at hand). We switched to this from an anti-MHC Class I already directly conjugated to PE, as this one is more comparable to how our samples are typically analyzed (with an initial murine anti-whatever, followed by GAM-PE).

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometry55555
    LuminexNot Rated
    EMNot Rated
  • Monoclonal Anti-HLA Class I Antigen antibody produced in mouse, clone W6/32, H1650 Sigma-Aldrich
    Used 5µL to stain 10^6 human CEA-positive MKN45 gastric carcinoma cells for 30 minutes prior analysis.
    Very good results alone with GAM-PE pAb.

    Western blotNot Rated
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometry55555
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex
EM