Works well in Western blots detecting SorCS1 from CHO-cells overexpressing either murine or human SorCS1.
Does NOT detect endogenous SorCS1 from p0 and adult mouse brains. A sorcs1-/- brain lysate was used as neg. reference. Cell expressing sorCS1 was used as positive ref.
WB 1:500
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On October 30, 2012Jenny BF wrote:
0
Rabbit polyclonal anti-sol-SorCS1 custom made at Dako, F7050, 5420.
This antibody was used for staining of CHO-cells stable expressing mouse SorCS1 variant c. It stains specific, but the background is also high in untransfected cells at 1:100 concentration.
On April 12, 2012Simon Glerup, Århus wrote:
4
Works nicely for IP of human SorCS1 expressed in CHO cells
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 25, 2012Anja A, Aarhus wrote:
1
Custommade antibody raised against the extracellular domain of SorCS1.
We used 5 ug/ml for staining of mouse primary hippocampal neurons.
On December 10, 2014 Peter Lund Ovesen wrote:
Works well in Western blots detecting SorCS1 from CHO-cells overexpressing either murine or human SorCS1.
Does NOT detect endogenous SorCS1 from p0 and adult mouse brains. A sorcs1-/- brain lysate was used as neg. reference. Cell expressing sorCS1 was used as positive ref.
WB 1:500
On October 30, 2012 Jenny BF wrote:
Rabbit polyclonal anti-sol-SorCS1 custom made at Dako, F7050, 5420.
This antibody was used for staining of CHO-cells stable expressing mouse SorCS1 variant c. It stains specific, but the background is also high in untransfected cells at 1:100 concentration.
On April 12, 2012 Simon Glerup, Århus wrote:
Works nicely for IP of human SorCS1 expressed in CHO cells
On February 25, 2012 Anja A, Aarhus wrote:
Custommade antibody raised against the extracellular domain of SorCS1.
We used 5 ug/ml for staining of mouse primary hippocampal neurons.
Not convincing.