Gad65, mouse mAb, ImmuQuest #IQ442

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3 User Reviews

  • Mouse hippocampal lysates, 30 ug protein / well.

    This Ab shows a specific band at the expected size of GAD2/GAD65 (65 kDa)

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • GAD65, MOUSE MAB, IMMUQUEST #IQ442
    I have used this antibody to do western blot on mouse frozen tissue. I have incubated with this antibody (1ug/ml) at 4 °C over night, then with secondary antibody at room temperature for 1 hour. The antibody gives nice band.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • mouse monoclonal Gad65 (IQ442) from ImmuQuest.

    This antibody was tested on lysate from adult mouse hippocampus and liver (picture). 50 ug protein was loaded in each lane. manufacturer suggests determining the optimal dilution by titration but recommends starting with 1ug/ml, so for this experiment i used a concentration of 1 ug/ml.
    The antibody was left over night at 4°C in a blocking buffer. The secondary antibody was left on for 1 hour at room temperature.

    This antibody is excellent for detecting GAD65 by western blotting! A clear band was observed in the hippocampus-sample while nothing was seen in the liver, as expected.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex