Related topics: Alphabetical list, Cellular marker proteins, Neurotransmission
See list of additional PSD95 antibodies at Linscott’s Directory
On December 4, 2019
Takahito Higashi, The University of Tokyo wrote:
This antibody works with immunostaining for identyfing post synaptic densities in a primary hippocampal neuron in a dillution 1:100.
For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).
On April 18, 2017
Anders Dalby wrote:
Did not work for immunostaining mouse hippocampal neurons. Clusters of PSD95 was not visible.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:500 dilution in blocking buffer.
This antibody was tested simultaneously with other antibodies for PSD95 (see below) and proved to be the worst.
PSD95 124 014 (Synaptic Systems) – Guniea pig
PSD95 p246 (Sigma) – Mouse (BEST)
PSD95 MAB 1596 (Chemicon/Millipore) – Mouse
On February 19, 2012
Simon Glerup, Aarhus wrote:
Does not work in our hands. We have tested it on synaptosomal preparations from the hippocampus and for staining of primary mouse hippocampal neurons.
IF dilution 1:50