Worked fine for immunostaining mouse hippocampal neurons. PSD95 clusters were visible but had a high background signal.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:100 dilution in blocking buffer.
This antibody was tested simultaneously with other antibodies for PSD95 (see below) and proved to be the second best.
PSD95 124 014 (Synaptic Systems) – Guniea pig
PSD95 p246 (Sigma) – Mouse (BEST)
PSD95 MA1-046 (ThermoFisher Scientific) – Mouse
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On October 6, 2015Simon Mølgaard wrote:
4
Works great for detecting PSD95 clusters in cultured mouse hippocampal neurons. The neurons were fixed in 4%PFA for 20 minutes.
The antibody was used at a dilution of 1:100
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 19, 2012Simon Glerup, Aarhus wrote:
4.5
Good antibody in Western blot. Recognizes a the protein of 95 kDa very specifically.
We have used for synaptosomal preparations of hippocampus where PSD95 is highly enriched in the post synaptic density fractions.
It also works great for staining cultured mouse hippocampal neurons (DIV14) as shown in the image above. PSD95 (red) and SorLA (green).
On April 18, 2017 Anders Dalby wrote:
Worked fine for immunostaining mouse hippocampal neurons. PSD95 clusters were visible but had a high background signal.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:100 dilution in blocking buffer.
This antibody was tested simultaneously with other antibodies for PSD95 (see below) and proved to be the second best.
PSD95 124 014 (Synaptic Systems) – Guniea pig
PSD95 p246 (Sigma) – Mouse (BEST)
PSD95 MA1-046 (ThermoFisher Scientific) – Mouse
On October 6, 2015 Simon Mølgaard wrote:
Works great for detecting PSD95 clusters in cultured mouse hippocampal neurons. The neurons were fixed in 4%PFA for 20 minutes.
The antibody was used at a dilution of 1:100
On February 19, 2012 Simon Glerup, Aarhus wrote:
Good antibody in Western blot. Recognizes a the protein of 95 kDa very specifically.
We have used for synaptosomal preparations of hippocampus where PSD95 is highly enriched in the post synaptic density fractions.
It also works great for staining cultured mouse hippocampal neurons (DIV14) as shown in the image above. PSD95 (red) and SorLA (green).
WB dilution 1:200
IF dilution 1:100