Related topics: Alphabetical list, Drosophila, Epitope tags
See list of additional GFP antibodies at Linscott’s Directory
On June 16, 2020
Bing Zhang initiative wrote:
Works great for ICC on fly brains and labella. Dilution: 1:1000.
On May 25, 2020
Xi Zha wrote:
This antibody worked great for immunostaining of perfusion-fixed mice brain slices for IF.
Dilution used: 1:1000
For more information see Zha et al. Cell Reports 2020, “VMHvl-Projecting Vglut1+ Neurons in the Posterior Amygdala Gate Territorial Aggression”.
On May 15, 2020
Jimena Berni, University of Cambridge wrote:
Great antibody for immunostaining.
Dilution used: 1:2000
On April 28, 2020
Justin Botterill, The Nathan S. Kline Institute for Psychiatric Research wrote:
Immunostaining were done on 4% paraformaldehyde perfusion-fixed brains that were cut at 50µm on a vibratome.
Antibody specificity is good and results in robust labeling. Can use relatively dilute concentrations, depending on signal (e.g., 1:2,000 to 1:10,000)
For more information see Botterill et al. Cell Reports 2019, “An Excitatory and Epileptogenic Effect of Dentate Gyrus Mossy Cells in a Mouse Model of Epilepsy”
On April 24, 2020
Huda Zoghbi, Baylor College of Medicine wrote:
Great antibody for IF.
Dilution used: 1:800
For more information see Lavery et al. eLife 2020, “ Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
On April 12, 2020
Wei Li, University of Chinese Academy of Sciences, Beijing wrote:
This antibody worked great for immunostaining.
Dilution used: 1:500
For more information see Cui et al. Cell Reports 2019, “Derivation of Mouse Haploid Trophoblast Stem Cells”.
On October 17, 2019
David I. Hughes, University of Glasgow wrote:
This antibody worked great for immunostaining of perfusion-fixed mice tissue for fluorescence microscopy.
Dilution used: 1:1000
For more information see Boyle et al., Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia, Cell Reports, vol 28, issue 2, p526-540, July 9, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30806-X).
On November 24, 2015
Sulieman Minhas wrote:
Anti-GFP Chicken antibody ab13970 Abcam
We have been using this particular antibody for many years and almost 8 different batches. for immunostainings, never had any complaints and all the batches work the same.