On January 18, 2016Camilla Gustafsen, Aarhus University wrote:
1
We have received an e-mail from Abcam with the following information regarding anti-LDLR antibody ab175883:
“Recent retests of ab175883 in western blot (WB) on mouse: lung, liver, colon and on human: liver showed negative results (i.e. no band). A band at the correct molecular weight was detected in HepG2 and HeLa cell lines but with high background. Therefore we have concluded that ab175883 is not specific to LDL Receptor and we have now withdrawn it from sale”
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 18, 2015Ditte Olsen, Aarhus University wrote:
2
Anti-LDLR Rabbit polyclonal abtibody, abcam175883
I have used this antibody diluted 1:1000 for WB detection of endogenous LDLR in HepG2 cells after stimulation with PCSK9. Although faint bands were observed of expected size, the antibody failed to detect PCSK9 induced reduction of LDLR.
On January 18, 2016 Camilla Gustafsen, Aarhus University wrote:
We have received an e-mail from Abcam with the following information regarding anti-LDLR antibody ab175883:
“Recent retests of ab175883 in western blot (WB) on mouse: lung, liver, colon and on human: liver showed negative results (i.e. no band). A band at the correct molecular weight was detected in HepG2 and HeLa cell lines but with high background. Therefore we have concluded that ab175883 is not specific to LDL Receptor and we have now withdrawn it from sale”
On November 18, 2015 Ditte Olsen, Aarhus University wrote:
Anti-LDLR Rabbit polyclonal abtibody, abcam175883
I have used this antibody diluted 1:1000 for WB detection of endogenous LDLR in HepG2 cells after stimulation with PCSK9. Although faint bands were observed of expected size, the antibody failed to detect PCSK9 induced reduction of LDLR.