Antibody: anti-myc epitope tag Rabbit IGG Ab00100-23.0 [9E10] (Absolute antibody)
The antibody was used to visualize myc-tagged proteins in transfected primary hippocampal neurons. Due to a high background signal, we were unable to distinguish the negative control from positive.
A concentration of 2ug/mL was used.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 6, 2015Camilla Gustafsen, Aarhus University wrote:
I have used this antibody (1:1000) for WB detection of 1µg Myc-tagged protein. A very strong band was seen. As negative control was used un-tagged protein. I am sure that the antibody can be diluted more, and less protein can be loaded.
On December 29, 2017 Anders Dalby, Aarhus University wrote:
Antibody: anti-myc epitope tag Rabbit IGG Ab00100-23.0 [9E10] (Absolute antibody)
The antibody was used to visualize myc-tagged proteins in transfected primary hippocampal neurons. Due to a high background signal, we were unable to distinguish the negative control from positive.
A concentration of 2ug/mL was used.
On November 6, 2015 Camilla Gustafsen, Aarhus University wrote:
Myc epitope tag
clone 9E10
mAb, rabbit IgG
Absolute Antibody
Ab00100-23.0
I have used this antibody (1:1000) for WB detection of 1µg Myc-tagged protein. A very strong band was seen. As negative control was used un-tagged protein. I am sure that the antibody can be diluted more, and less protein can be loaded.
2. Ab: HRP-anti Rabbit IgG, Cell Signaling 7074S (1:2000), 1 hour at RT
Similar band-intensity was seen with anti-Myc tag antibodies Ab00100-1.1 and Ab00100-2.0