On October 5, 2015Veerle Paternoster, Aarhus University wrote:
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anti-CREB1, Mouse Monoclonal, Novus Biological #NB100-56525SS
Tested for Western blotting on 100 ug total cell lysate from HEK cells, mouse brain and pig brain. used dilution: 1/2000 (recommended).
Gives a very clear band at expected size (43 kDa) in all extracts. Highly overexposed, would dilute more! Some fainter bands just below, but they may disappear when appropriate dilution is used.
On October 5, 2015 Veerle Paternoster, Aarhus University wrote:
anti-CREB1, Mouse Monoclonal, Novus Biological #NB100-56525SS
Tested for Western blotting on 100 ug total cell lysate from HEK cells, mouse brain and pig brain. used dilution: 1/2000 (recommended).
Gives a very clear band at expected size (43 kDa) in all extracts. Highly overexposed, would dilute more! Some fainter bands just below, but they may disappear when appropriate dilution is used.