M6PR (cation independent), rabbit pAb, Abcam, ab32815

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One User Review

  • Producer: Abcam
    Catalog No: ab32815
    Used dilution: 1:100 (IF); 1:100 (WB)
    Cell line: primary porcine brain endothelial cells; primary porcine pericytes; MDCK
    Host organism: Rabbit
    Character: primary, polyclonal

    Antibody works good in primary porcine brain endothelial cells, primary pericytes and ok in MDCK at indicated dilution for IF. Note that porcine and canine are predicted reactivity species by manufacturer.
    In WB few additional bands appeared on blot. For IF as a secondary antibody Life technologies goat anti-mouse Alexa Fluor 568 (1:350 or 1:200) was used.

    Cells fixed with 4% PFA, permeabilized with 0.1% Triton-X100, blocked with 2% BSA.

    Western blot22222
    Immunostaining33333
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex
EM