Phospho-GSK-3β (Ser9), rabbit pAb, Cell Signaling Technology, 9336

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3 User Reviews

  • We have used this antibody for western blotting with great succes. 
    For more information see Aras et al., 2019, Cell Reports 27, 2385–2398 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30573-X)

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • Phospho-GSK-3β (Ser9) Antibody #9336
    Cell Signaling Technology

    This antibody has regularly given a clear and specific band at expected molecular weight when detecting GSK3β phosphorylation in mouse hippocampal and cortical lysates in Western blot.

    The antibody is diluted 1:1000 in 3% BSA in TBS-T + 1:1000 NaN3. Dilution has given a good signal for 5-7 uses when stored at 4 degrees.

    Incubation overnight at 4 degrees
    Washing steps with TBS-T
    Secondary antibody diluted in 5% milk powder in TBS-T for 1 hour
    Detection with ECL+ (ThermoScientific)

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated
  • I have used anti-phosphoGK3beta Ser9 (CST #9336) at 1:1000 on murine heart tissue. Gives a nice band at 45 kDa as expected.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex
EM