SOX2, mouse mAb (clone 4G8), Novus Biologicals, NBP2-29623

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One User Review

  • Tested in neural progenitor cells, there was no specific signal, the band is very weak even though after long time exposure. And there is some background.

    Primary antibody: 1:1000 dilution in 5%milk/TBST buffer, incubate in RT for 1 hour.
    Secondary antibody: anti-mouse IgG-HRP, 1:10000 dilution in 5%milk/TBST buffer, incubate inRT for 1 hour.
    Image the signal using ImageQuant for exposure time of 10-15min.

    Western blot22222
    ImmunostainingNot Rated
    IPNot Rated
    ChIPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ChIP
ELISA
Flow cytometry
Luminex
EM