GAPDH, mouse mAb (clone 1D4), Novus Biologicals, NB300-221

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One User Review

  • This antibody is excellent to be chosen as an internal control for western blot. I have tested both in neural progenitor cell and medulloblastoma cell. Both cell line the signal is very clear, sharp, specific, no background.
    Primary antibody: 1:1000 dilution in 5%milk/TBST buffer, incubate in RT for 1 hour.
    Secondary antibody: anti-mouse IgG-HRP, 1:10000 dilution in 5%milk/TBST buffer, incubate inRT for 1 hour.
    Image the signal using ImageQuant for exposure time of 1-3 sec.
    See image above.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
    EMNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex
EM