This antibody detects MAKP in rat primary Schwann cell lysates(50 ug). Dilution 1:1000. It appears to be very specific for MAPK, as I only see two lanes (app. 42 kDa and 44 kDa) in my blot.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On March 22, 2015Jakob Vejby Larsen, Aarhus University wrote:
5
I have used this antibody in murine primary macrophages and 293 cells. It is very good! Dilution 1:1000
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On May 9, 2014Mads Fuglsang Kjolby wrote:
5
WB 1:1000 in primary murine macrophages.
OBS: This antibody is against MAPK and NOT phospho MAPK! (Cell Signaling 4695)
Western blot
Immunostaining
Not Rated
IP
Not Rated
ChIP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On February 3, 2012Mette R wrote:
4
WB dilution 1:1000 on mouse spinal cord tissue
100 ug per lane
Use only once!
On May 5, 2020 Margie Sutton, University of Texas MD Anderson Cancer Center wrote:
WB dilution 1:2000
IF rating: 5 stars
IHC rating: 4 stars
For more information see Sutton et al. Cell Reports 2019, “DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters”
https://www.sciencedirect.com/science/article/pii/S221112471931527X#sec5
On March 26, 2015 Maj Ulrichsen wrote:
This antibody detects MAKP in rat primary Schwann cell lysates(50 ug). Dilution 1:1000. It appears to be very specific for MAPK, as I only see two lanes (app. 42 kDa and 44 kDa) in my blot.
On March 22, 2015 Jakob Vejby Larsen, Aarhus University wrote:
I have used this antibody in murine primary macrophages and 293 cells. It is very good! Dilution 1:1000
On May 9, 2014 Mads Fuglsang Kjolby wrote:
WB 1:1000 in primary murine macrophages.
OBS: This antibody is against MAPK and NOT phospho MAPK! (Cell Signaling 4695)
On February 3, 2012 Mette R wrote:
WB dilution 1:1000 on mouse spinal cord tissue
100 ug per lane
Use only once!