Ab Name: CD71 (K-20) Transferrin Receptor
Producer: Invitrogen (Bought at Thermo Fisher)
Catalog No: 13-6800
Host organism: Mouse
Used dilution: 1:1000 (WB) – 0.1 ug/ml
Secondary Ab: Anti-Mouse HRP, Cell Signaling: 7076 (1:2000)
Cell line: primary porcine brain endothelial cells (pBEC), HEK 293, primary rat astrocyttes (rAstro) – 5 ug
In both pBEC and HEK 293 lysate the antibody produced a single clear band at the appropriate size. In rAstro the band was at the same size, but must weaker
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On May 8, 2015Mathilde Bonnemaison, University of Nebraska Medical Center wrote:
On August 22, 2016 Niels M Kristiansen wrote:
Ab Name: CD71 (K-20) Transferrin Receptor
Producer: Invitrogen (Bought at Thermo Fisher)
Catalog No: 13-6800
Host organism: Mouse
Used dilution: 1:1000 (WB) – 0.1 ug/ml
Secondary Ab: Anti-Mouse HRP, Cell Signaling: 7076 (1:2000)
Cell line: primary porcine brain endothelial cells (pBEC), HEK 293, primary rat astrocyttes (rAstro) – 5 ug
In both pBEC and HEK 293 lysate the antibody produced a single clear band at the appropriate size. In rAstro the band was at the same size, but must weaker
On May 8, 2015 Mathilde Bonnemaison, University of Nebraska Medical Center wrote:
Transferrin receptor antibody
Invitrogen
Clone H68.4
Cat. #13-6800
Host: mouse