On March 22, 2015Britt R. Ardal (THJ lab, Aarhus University) wrote:
4
Used for immune fluorescence on Hek293 cells 1:500.
signal was clear and easy to do microscopy on when using Alexa 594 (texas red) secondary antibody in 1:1000.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On March 22, 2015Britt R. Ardal (THJ lab, Aarhus University) wrote:
4
Used for Western blotting in 1:5000 in 5% milk o/n on rotation at 4 degrees.
Signal was very good and clear with no unspecific binding.
Antibody targets the c-terminus of NOP56.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
EM
Not Rated
On March 22, 2015Ewa Molska (THJ lab, Aarhus University) wrote:
4
The antibody detects a ~70 kDa WB band. Gives strong clean signal (diluted 1:5000) and can be reused
On March 22, 2015 Britt R. Ardal (THJ lab, Aarhus University) wrote:
Used for immune fluorescence on Hek293 cells 1:500.
signal was clear and easy to do microscopy on when using Alexa 594 (texas red) secondary antibody in 1:1000.
On March 22, 2015 Britt R. Ardal (THJ lab, Aarhus University) wrote:
Used for Western blotting in 1:5000 in 5% milk o/n on rotation at 4 degrees.
Signal was very good and clear with no unspecific binding.
Antibody targets the c-terminus of NOP56.
On March 22, 2015 Ewa Molska (THJ lab, Aarhus University) wrote:
The antibody detects a ~70 kDa WB band. Gives strong clean signal (diluted 1:5000) and can be reused