Related topics: Alphabetical list, Epitope tags
See list of additional GFP antibodies at Linscott’s Directory
On November 19, 2019
Hao Wang, Zhejiang University School of Medicine wrote:
I have used this antibody for in situ hybridization in a dilution of 1:500. This antibody works so well for me.
For more information see Hao et al., The Lateral Hypothalamic and BNST GABAergic Projections to the Anterior Ventrolateral Periaqueductal Gray Regulate Feeding, Cell Reports, vol 28, issue 3, p616-624, July 16, 2019 (https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30824-1#%20).
On November 2, 2017
Julie Torvund-Jensen wrote:
I have used this antibody to detect GFP in transiently transfected HepG2 cells.
It gives a very nice and specific signal. Dilution 1:2000.
On July 13, 2016
Signe Petersen, Aarhus University wrote:
Anti-GFP antibody (ab6556) ABCAM
I used this antibody for western blotting in a dilution of 1:2000 with great results. I blotted cell lysates from primary hippocampal neurons. The signal was not very powerful, however. No unspecific bands were detected.
On March 2, 2016
Signe Petersen wrote:
I used this antibody on GFP-transfected, cultured mouse hippocampal neuron lysates at a dilution of 1:2000.
I conducted a western blot with excellent results.
On October 20, 2015
Simon Mølgaard wrote:
This antibody works excellent for detecting GFP by western blot!
I used it on lysates from cultured mouse hippocampal neurons that had been transfected with GFP.
I used it at a dilution of 1:2000
On November 12, 2014
Sune Skeldal, Aarhus University wrote:
I have used this rabbit polyclonal antibody for western blotting with great success. It is sensitive not only to YFP but (according to the company) also other variants such as GFP and CFP. I use a dilution of 1:2000.