F4/80, chicken pAb, ImmuQuest IQ605

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F480 Iba1
Mouse cortical glia stained with anti-F4/80 (green), anti-Iba1 (Wako 019-19741, red), and Hoechst (blue)
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See list of additional F4/80 antibodies at Linscott’s Directory

One User Review

  • F4/80 is known as a marker for macrophages and microglia.

    We used this antibody to stain primary mouse cortical glia and the signal was indeed present in cells also positive for Iba1 (see above). However, the staining was also present in a number of other cells not positive for Iba1.
    Cells were permeabilized in PBS+0.1% TritonX100 and blocked in PBS+10% FBS.
    Dilution for IF 2 ug/ml

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex