Parvalbumin, mouse mAb clone 58E1, oyster 488 labeled, Synaptic Systems Cat. No. 195 011C2

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3 User Reviews

  • Single fluorescent staining targeting Parvalbumine expressing interneurons.

    Primary antibody: Mouse Mab Clone 58E1, OYSTER-488-LABELED [Synaptic Systems, cat: 195 011C2], 1:200 and 1:600 dilution.

    Protocol in short:
    Sections were washed (3x10min), treated with target retrieval solution and washed again (1×10 min) Sections were then rinsed in FAB blocking buffer with Donkey anti-mouse followed by incubation with a fluorescent 488-labeled antibody directed towards PV (SYNAPTIC SYSTEMS) for 5 days. Sections were then washed (3×10 min) and (1×10 min) in DAPI and mounted on slides, air-dried and then mounted with cover slips.

    Results:
    Staining appears specific, as PV staining is intense around cell nuclei stained with DAPI. The PV antibody is thus appropriate for staining PV interneurons. The staining results further shows that both the 1:200 and 1:600 concentration are suitable.

    Western blot55555
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Double staining targeting Parvalbumine expressing interneurons.

    Primary antibody: Mouse Mab Clone 58E1, OYSTER-488-LABELED [Synaptic Systems, cat: 195 011C2]

    Secondary antibody: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 568 conjugate [Life technologies Cat: A10042]

    Protocol in short:
    In short, sections were washed (3x10min), treated with target retrieval solution and washed again (1×10 min). Sections were then rinsed in FAB blocking buffer with Donkey anti-mouse followed by incubation with a rabbit antibody directed towards GABA for 2 days. Sections were then washed (3×10 min) and incubated for 4 hours with a secondary antibody, Donkey-anti-Rabbit with a fluorescent 568-labeled directed towards the primary GABA antibody. The second primary antibody 488-labeled directed towards PV was added and sections were incubated for 5 days. Sections were then washed (4×15 min) and (1×10 min) in DAPI. Finally, the sections were mounted on slides, air-dried and then mounted with cover slips.

    Results:
    The double staining results show that the double staining with both antibodies at the same time was not successful. The single stainings appear to be specific, but when both antibodies are combined, the GABA antibody loses its specificity.

    Western blotNot Rated
    Immunostaining22222
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody is primary labelled to oyster 488. It has been used on cryo-sections with a thickness up to 150 um with great succes. This antibody shows high specificity with very little background.

    I used this at a concentration of 1:200.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
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Luminex