Goat anti-mouse Alexa 488, Invitrogen cat. A-11001

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Fig 2 Kernohan
Kernohan et al. used this antibody in MethodsX 2014. The figure from the paper shows confocal images of dual labelled DNA FISH in fixed mouse brain sections. (a) Example of field of view of neonatal mouse brain. Each cell has two foci per probe marking the two alleles. (b) Example of a collapsed image showing nuclear DNA foci and (c) its corresponding three dimensional rendering conducted with the Volocity software package. This data can be used to measure relative distances, signal volume or number of foci within the cell.

2 User Reviews

  • Goat polyclonal anti-mouse IgG-Alexa-488 – Invitrogen/Life Technologies/Thermo Scientific (Cat. No. – A-11001).

    Worked well on frozen human skin sections (50-micrometer thick) in 1:1000 for 3 hours.

    Western blot55555
    ImmunostainingNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Secondary antibody works very well for all immunofluorescence techniques. Used on fixed brain sections (fixed in 4% PFA) at 1/1000.

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Western blot
Immunostaining
ELISA
Flow cytometry
Luminex