Worked very well for immunostaining mouse hippocampal neurons.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:1000 dilution in blocking buffer.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On April 13, 2014Simon Mølgaard, Aarhus University wrote:
4
We have tested this on cultured primary hippomcampal neurons and this works very well for staining against MAP2.
On April 18, 2017 Anders Dalby wrote:
Worked very well for immunostaining mouse hippocampal neurons.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:1000 dilution in blocking buffer.
On April 13, 2014 Simon Mølgaard, Aarhus University wrote:
We have tested this on cultured primary hippomcampal neurons and this works very well for staining against MAP2.
We used this at a concentration of 1:1000.