Related topics: Apoptosis
See list of additional Cleaved Caspase-3 antibodies at Linscott’s Directory
On April 4, 2020
Ana Jovicic, Genentech wrote:
IHC was performed on fixed frozen mouse muscle tissue. The antibody worked great.
Dilution used: 1:200
For more information see Yartseva et al. Cell Reports 2020, “ Heterogeneity of Satellite Cells Implicates DELTA1/NOTCH2 Signaling in Self-Renewal”.
On July 1, 2019
Sérgio Almeida wrote:
This antibody has worked quite well for the detection of cleaved Caspase-3 in lysates of primary mouse neuronal cultures by WB (1:1000).
On September 11, 2017
Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University wrote:
This antibody was used for immunofluorescent staining of paraffin-embedded sections of PFSK-1 and D-283 tumor xenografts.
Dilution: 1:100 in PBS or in blocking/permeabilization buffer (buffer containing 5% normal goat serum (NGS) and 0.1-0.3% Triton-X 100 in 1xPBS)
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
On October 19, 2015
Sara Konstantin Nissen wrote:
Caspase-3 (8G10) Rabbit mAb #9665 Cell signaling
This AB is suppose to detect pro-caspase3 as well as celaved caspase-3. However, I only maneged to detect pro-caspase3 in primary blood purified CD4+ T cells ( treated with stausporine). One fine band for the pro-caspase3, but no band for the celeaved active capse3, therefor only 2 stars. Remeber to block in skim milk.
On January 16, 2012
Simon Glerup, Aarhus wrote:
We have used this antibody in immunofluorescence on sections of sciatic nerve and dorsal root ganglions following injury.
There is a nice increase in activated caspase-3 staining in the DRG of the injured side. And also in the fiber in Schwann cells closed to the site of injury. This increase is not seen in the uninjured side.
The antibody only gets three stars because there appears to be some batch variations for this antibody. One of the batches we have acquired may show cross reactivity with uncleaved caspase-3.