Cleaved Caspase-3 (Asp175) rabbit anti-human Cell Signaling 9661

  • 33333
  • 44444
  • No Ratings
  • No Ratings
  • No Ratings
  • No Ratings

Related topics:

4 User Reviews

  • This antibody has worked quite well for the detection of cleaved Caspase-3 in lysates of primary mouse neuronal cultures by WB (1:1000).

    Western blot44444
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • This antibody was used for immunofluorescent staining of paraffin-embedded sections of PFSK-1 and D-283 tumor xenografts.

    Dilution: 1:100 in PBS or in blocking/permeabilization buffer (buffer containing 5% normal goat serum (NGS) and 0.1-0.3% Triton-X 100 in 1xPBS)

    Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Caspase-3 (8G10) Rabbit mAb #9665 Cell signaling

    This AB is suppose to detect pro-caspase3 as well as celaved caspase-3. However, I only maneged to detect pro-caspase3 in primary blood purified CD4+ T cells ( treated with stausporine). One fine band for the pro-caspase3, but no band for the celeaved active capse3, therefor only 2 stars. Remeber to block in skim milk.

    Western blot22222
    ImmunostainingNot Rated
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • We have used this antibody in immunofluorescence on sections of sciatic nerve and dorsal root ganglions following injury.

    There is a nice increase in activated caspase-3 staining in the DRG of the injured side. And also in the fiber in Schwann cells closed to the site of injury. This increase is not seen in the uninjured side.

    The antibody only gets three stars because there appears to be some batch variations for this antibody. One of the batches we have acquired may show cross reactivity with uncleaved caspase-3.

    Dilution 1:200

    Western blotNot Rated
    Immunostaining33333
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

Leave a review

Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex