Great antibody for WB of human PCSK9 (dilution 1:1500). In my experience, it can be frozen and re-used 3-4 times.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 15, 2016Sune Skeldal, Aarhus University wrote:
4
Good results in western blotting (1:1500) detecting purified PCSK9.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 4, 2015Camilla Gustafsen, Aarhus University wrote:
5
I have used this antibody to detected PCSK9 (WT and 6His-tagged) secreted to the media of stably transfected HEK293 cells. WB inserted
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On September 14, 2015Camilla Gustafsen, Aarhus University wrote:
5
This antibody is excellent for WB detection of human PCSK9 in media and lysate from transfected CHO, HepG2 and Hek 239 cells. We dilute 1:1000 and re-use several times.
The antibody does not recognize murine PCSK9
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On May 26, 2013Camilla Gustafsen wrote:
5
Very good for WB detection of hPCSK9 in stably transfected HepG2 cells. Detects both mature and proform of PCSK9. Can be frozen/thawed after dilution and re-used several times.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 23, 2012Anja A, Aarhus wrote:
2
This antibody only works very poorly for immunoprecipitation of human PCSK9.
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 29, 2012Anja A, Aarhus wrote:
3
Excellent for Western blotting. Does not work for immunofluorescense.
We have tested it on 293 cells transfected with human PCSK9 expression vector.
On December 8, 2017 Julie Torvund-Jensen wrote:
Great antibody for WB of human PCSK9 (dilution 1:1500). In my experience, it can be frozen and re-used 3-4 times.
On March 15, 2016 Sune Skeldal, Aarhus University wrote:
Good results in western blotting (1:1500) detecting purified PCSK9.
On November 4, 2015 Camilla Gustafsen, Aarhus University wrote:
I have used this antibody to detected PCSK9 (WT and 6His-tagged) secreted to the media of stably transfected HEK293 cells. WB inserted
On September 14, 2015 Camilla Gustafsen, Aarhus University wrote:
This antibody is excellent for WB detection of human PCSK9 in media and lysate from transfected CHO, HepG2 and Hek 239 cells. We dilute 1:1000 and re-use several times.
The antibody does not recognize murine PCSK9
On May 26, 2013 Camilla Gustafsen wrote:
Very good for WB detection of hPCSK9 in stably transfected HepG2 cells. Detects both mature and proform of PCSK9. Can be frozen/thawed after dilution and re-used several times.
On March 23, 2012 Anja A, Aarhus wrote:
This antibody only works very poorly for immunoprecipitation of human PCSK9.
On February 29, 2012 Anja A, Aarhus wrote:
Excellent for Western blotting. Does not work for immunofluorescense.
We have tested it on 293 cells transfected with human PCSK9 expression vector.