Rab7 (D95F2) XP® Rabbit mAb #9367
This antibody works great to detect Rab7 in primary cortical neuronal cultures from mice. A very clear signal was obtained with little to no background signal.
The neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:100 dilution in blocking buffer o.n.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On February 19, 2014Morten S. Nielsen, Aarhus University wrote:
1
Rabbit anti Rab7 from Cell Signalling (#9367) were tested for immunefluorescence in HEK293 and SHSY5Y cells. Staining were made according to Cell Signalling’s recommendations, but poor results were obtained.
On September 23, 2018 Anders Dalby, Aarhus University, Denmark wrote:
Rab7 (D95F2) XP® Rabbit mAb #9367
This antibody works great to detect Rab7 in primary cortical neuronal cultures from mice. A very clear signal was obtained with little to no background signal.
The neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:100 dilution in blocking buffer o.n.
On February 19, 2014 Morten S. Nielsen, Aarhus University wrote:
Rabbit anti Rab7 from Cell Signalling (#9367) were tested for immunefluorescence in HEK293 and SHSY5Y cells. Staining were made according to Cell Signalling’s recommendations, but poor results were obtained.