On October 25, 2013Morten Nielsen, Aarhus University wrote:
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Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells. Did not work very well under tested conditions.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
Picture above shows SHSY5Y cells stained using this antibody (green). Nuclei are stained blue using Hoechst.
On October 25, 2013 Morten Nielsen, Aarhus University wrote:
Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells. Did not work very well under tested conditions.
Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.
Picture above shows SHSY5Y cells stained using this antibody (green). Nuclei are stained blue using Hoechst.