HSPA8 goat pAb Everest Biotech EB07295

  • 11111
  • 1.51.51.51.51.5
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2 User Reviews

  • This antibody did not work for me in both immunostaining and WB.

    IF: Primary hippocampal neruons from mice were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a both 0,5ug/mL, 1ug/mL and 2ug/mL dilution in blocking buffer. No clear signal was observed using these concentrations.
    WB: I used 1:5000 dilution of primary hippocampal neurons and was unable to detect HSPA8.

    Western blot11111
    Immunostaining11111
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • Was tested for Immunofluorescence in a concentration of 1µg/ml on HEK293 and SHSY5Y cells.
    Weak signal in cytoplasm and nucleus was observed, corresponding to expected localisation. However, not optimal for fluorescent imaging.
    Cells were fixed in 4 % paraformaldehyde and permeabilised with 0.25 % saponine. Primary antibody was incubated 1 hour at RT.

    Western blotNot Rated
    Immunostaining22222
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex