Related topics: Alphabetical list, Cytoskeleton, Loading controls
See list of additional beta-actin antibodies at Linscott’s Directory
On January 10, 2020
Mikolaj Zaborowski, Harvard Medical School - Massachusetts General Hospital wrote:
This antibody worked great for western blotting.
For more information see Zaborowski et al. Cell Reports 2019, Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles
On March 14, 2019
Anders Dalby wrote:
This antibody works great to visualize the cytoskeleton of HEK293 cells. Dilution 1:1000.
On November 28, 2017
Anders Dalby wrote:
Very good antibody that gives a strong signal.
On September 11, 2017
Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University wrote:
This antibody was used as Western blot loading control for lysates of human CNS embryonal tumors, medulloblastomas and tumor cell lines D283, D324 and PFSK-1.
Dilution: 1:5000 (1x TBST with 5% w/v BSA)
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
On February 8, 2017
Niels Degn, Aarhus University wrote:
Works very well for loadning control for western blot on lysates from mouse striatum and cortex. Dilution 1:5000.
On December 15, 2016
Julie Torvund-Jensen wrote:
A excellent antibody for Western blots. I have diluted this antibody 1:10.000 and still get a very high, specific signal from HepG2 cells. The antibody solution can be reused several times.
On June 3, 2016
Camilla Gustafsen wrote:
I have used this antibody as af loading control for mouse liver and adrenal gland homogenates (20-60 µg total protein). I dilute 1:5000 and reuse the antibody several times
On March 16, 2016
Sune Skeldal, Aarhus University wrote:
This antibody works well in western blotting using 1:3000 dilution.
On February 26, 2016
Signe Petersen wrote:
I used this as a primary antibody for western blotting of cultured hippocampal neurons at a dilution of 1:5000 with excellent results.
On November 23, 2015
Rikke H Kofoed, Aarhus University wrote:
I routinely use this antibody as a western blotting loading control in a dilution of 1:5000. It gives a very strong band when using 10 ug cell lysate. I have tried it on rat cell cultures and human cell cultures as well as mouse primary neurons.
On November 13, 2015
Lars Meinertz Byg wrote:
I tried this antibody in a 1:1000 dilution in immunoprecipitation of Human b-actin (SY5Y-cells), with poor results. however, it works great for westernblotting in a 1:3000 solution, and can easily be reused.
On August 21, 2015
Simon Mølgaard wrote:
i have used this for wb loading control from mouse hippocampal tissue lysates. Works excellent for detection.
used at a dilution of 1:5000. Can be frozen and reused.
On October 4, 2013
Camilla Gustafsen, Aarhus University wrote:
We have used this antibody (1:2500) for WB loading control (HepG2 lysate).
On January 14, 2012
Anja A, Aarhus wrote:
Great antibody in Western blotting. Very high signal. In our Western blotting imaging system, we get a strong distinct within seconds.
Works also on stripped membranes. The diluted used antibody can also be frozen and used again.