On December 13, 2018Kasper Budolph Pedersen wrote:
4
This is an good antibody.
We have used it for for detection of LDLR, diluted 1:500 (Western Blot) and re-used several times.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On June 22, 2017Julie Torvund-Jensen wrote:
4
This antibody also works nicely for fluorescent immunostaining of mice adrenal gland tissue (paraffin). Working dilution 1:100.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On December 15, 2016Julie Torvund-Jensen wrote:
5
This antibody works great for Western blotting (diluted 1:2000) for detection of both murine and human LDLR.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 5, 2015Camilla Gustafsen, Aarhus University wrote:
5
This is an exceptional good antibody!
We have used it for detection of LDLR in mouse liver after iv administration of PCSK9. The antibody detects a single band of expected size (100 kDa). The identity of the band is confirmed by a clear PCSK9 induced down regulation of protein level, as several groups have published it (see Western blot above).
50 µg protein (membrane preparation) was loaded, and the antibody was diluted 1:2000 in 5% milk in TBS-T. We re-use the antibody several times (10+) and store it on ice in the cold room.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 21, 2015Laura Tvilling Poulsen (Aarhus University) wrote:
3
I used this antibody (1:100) for immunostaining of endogenous LDLR. A strong signal was detected in HepG2 cells, however a similar staining was seen in HepG2 cells with overexpression of PCSK9, a protein that induces degradation of lipoprotein receptors including LDLR.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On October 30, 2014Simon Glerup, Aarhus University wrote:
1
This antibody does not work for IP of endogenous LDL receptor from HEK293 cells.
We did IPs from metabolically labelled cells (S35)and saw a lot of unspecific bands. The antibody even pulled down the related receptor SorLA.
Western blot
Not Rated
Immunostaining
Not Rated
IP
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On September 17, 2014Wojciech Tobis wrote:
0
I did not used this antibody, but I’d like to spread the word, because science never has too much money to spend. Especially when you can buy ABs much cheaper.
So, this antibody is also available at:
Novus: NB110-57162
Genetex: GTX61553
Origene: TA300856 (you get the free sample of positive control with this one!)
It is exactly the same antibody, just look at the pictures and specs. What’s different? Price! Abcam: 410 euro, Novus: 379 euro.
One more thing: I have no connections with Novus, Origene, Abcam or any other company. I use antibodies daily, so I know a things or two.
On November 25, 2012Simon G wrote:
4
This LDL receptor antibody works nicely for Western blotting.
We used mouse liver homogenates as samples. The LDL receptor antibody detected a band of the expected size. However two unspecific bands of low molecular weight were also observed.
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 22, 2012Camilla Gustafsen wrote:
5
We have used this excellent antibody for immunostaining (1:100) of HepG2 cells, and for WB (1:3000) detection of hLDLR (HepG2) and mLDLR (liver homogenates). For WB we reuse the antibody several times.
On December 13, 2018 Kasper Budolph Pedersen wrote:
This is an good antibody.
We have used it for for detection of LDLR, diluted 1:500 (Western Blot) and re-used several times.
On June 22, 2017 Julie Torvund-Jensen wrote:
This antibody also works nicely for fluorescent immunostaining of mice adrenal gland tissue (paraffin). Working dilution 1:100.
On December 15, 2016 Julie Torvund-Jensen wrote:
This antibody works great for Western blotting (diluted 1:2000) for detection of both murine and human LDLR.
On November 5, 2015 Camilla Gustafsen, Aarhus University wrote:
This is an exceptional good antibody!
We have used it for detection of LDLR in mouse liver after iv administration of PCSK9. The antibody detects a single band of expected size (100 kDa). The identity of the band is confirmed by a clear PCSK9 induced down regulation of protein level, as several groups have published it (see Western blot above).
50 µg protein (membrane preparation) was loaded, and the antibody was diluted 1:2000 in 5% milk in TBS-T. We re-use the antibody several times (10+) and store it on ice in the cold room.
On March 21, 2015 Laura Tvilling Poulsen (Aarhus University) wrote:
I used this antibody (1:100) for immunostaining of endogenous LDLR. A strong signal was detected in HepG2 cells, however a similar staining was seen in HepG2 cells with overexpression of PCSK9, a protein that induces degradation of lipoprotein receptors including LDLR.
On October 30, 2014 Simon Glerup, Aarhus University wrote:
This antibody does not work for IP of endogenous LDL receptor from HEK293 cells.
We did IPs from metabolically labelled cells (S35)and saw a lot of unspecific bands. The antibody even pulled down the related receptor SorLA.
On September 17, 2014 Wojciech Tobis wrote:
I did not used this antibody, but I’d like to spread the word, because science never has too much money to spend. Especially when you can buy ABs much cheaper.
So, this antibody is also available at:
Novus: NB110-57162
Genetex: GTX61553
Origene: TA300856 (you get the free sample of positive control with this one!)
It is exactly the same antibody, just look at the pictures and specs. What’s different? Price! Abcam: 410 euro, Novus: 379 euro.
One more thing: I have no connections with Novus, Origene, Abcam or any other company. I use antibodies daily, so I know a things or two.
On November 25, 2012 Simon G wrote:
This LDL receptor antibody works nicely for Western blotting.
We used mouse liver homogenates as samples. The LDL receptor antibody detected a band of the expected size. However two unspecific bands of low molecular weight were also observed.
On November 22, 2012 Camilla Gustafsen wrote:
We have used this excellent antibody for immunostaining (1:100) of HepG2 cells, and for WB (1:3000) detection of hLDLR (HepG2) and mLDLR (liver homogenates). For WB we reuse the antibody several times.