SorLA antibody sheep anti-human/mouse R&D Systems AF5699

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4 User Reviews

  • Works very well for ICC on HepG2 cells transiently transfected with full-length human SorLA.
    Dilution 1:1000.

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I have tested this antibody on rat hippocampal neurons. It doesn’t work very well and gives a lot of background.
    Protocol details:
    Fixed with 4% PFA, 4% sucrose.
    Permeabilized with 0.02% Triton X-100 in gelatin
    Blocked with gelatin
    Antibody incubation: in gelatine, 30 min 37 degrees.
    The image above shows an example of a hippocampal neuron stained for MAP2(blue) and SorLA using AF5699 (red).

    Western blotNot Rated
    Immunostaining22222
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • I have used this antibody for immunostainings on adult mouse barin tissue as well as cultured hipoocampal neurons. In both cases this antibody worked very well for detection of SorLA.

    concentration 5ug/ml

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated
  • We have used this SorLA antibody for IHC on PFA-fixed cryosections of mouse brain treated with antigen retrieval kit.

    The antibody is great and sections from SorLA knockouts are blank confirming its specificity.

    Dilution: 5 ug/ml

    Western blotNot Rated
    Immunostaining55555
    IPNot Rated
    ELISANot Rated
    Flow cytometryNot Rated
    LuminexNot Rated

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Western blot
Immunostaining
IP
ELISA
Flow cytometry
Luminex