I have used this antibody on cryosections of mouse embryonic tissue. It stains very well, clearly delineating cells known to express calretinin. Very high signal to background contrast. Dilution used were 1:250 but 1:500 also works well.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On January 19, 2015Hande Login wrote:
5
I performed IHC with this antibody on 12 um cryosections of mouse olfactory bulb. I got very nice, specific staining of interneurons without any background.
Antibody dilution: 1:500
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On November 12, 2014Simon Mølgaard, Aarhus University wrote:
5
This was tested os hippocampal lysates from p20 mice.This showed a very nice staining with very litttle background. Used at 1:1000
Western blot
Immunostaining
Not Rated
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On August 8, 2014Maj Ulrichsen wrote:
5
I have used this antibody on spinal cord cryo-sections at a dilution of 1:500. It is an excellent antibody that shows intense staining of a dense band of cells in the superficial lamina and of some cells in the deeper laminae of the dorsal horn. It shows staining both in the nuclei and the cytoplasm of positive cells and has a low background staining.
Western blot
Not Rated
Immunostaining
IP
Not Rated
ELISA
Not Rated
Flow cytometry
Not Rated
Luminex
Not Rated
On March 29, 2012Simon Jensen, Aarhus wrote:
5
This antibody works very well for staining of tissue, both using DAB and fluorescence. At a concencration of 1:1000 very low background is seen.
Staining of hippocampal neurons in culture using a concentration of 1:1000 also showed a clear distinction between calretinin-positive and -negative cells. However, in this case the background is a little bit higher.
On September 23, 2015 Simon Boggild wrote:
I have used this antibody on cryosections of mouse embryonic tissue. It stains very well, clearly delineating cells known to express calretinin. Very high signal to background contrast. Dilution used were 1:250 but 1:500 also works well.
On January 19, 2015 Hande Login wrote:
I performed IHC with this antibody on 12 um cryosections of mouse olfactory bulb. I got very nice, specific staining of interneurons without any background.
Antibody dilution: 1:500
On November 12, 2014 Simon Mølgaard, Aarhus University wrote:
This was tested os hippocampal lysates from p20 mice.This showed a very nice staining with very litttle background. Used at 1:1000
On August 8, 2014 Maj Ulrichsen wrote:
I have used this antibody on spinal cord cryo-sections at a dilution of 1:500. It is an excellent antibody that shows intense staining of a dense band of cells in the superficial lamina and of some cells in the deeper laminae of the dorsal horn. It shows staining both in the nuclei and the cytoplasm of positive cells and has a low background staining.
On March 29, 2012 Simon Jensen, Aarhus wrote:
This antibody works very well for staining of tissue, both using DAB and fluorescence. At a concencration of 1:1000 very low background is seen.
Staining of hippocampal neurons in culture using a concentration of 1:1000 also showed a clear distinction between calretinin-positive and -negative cells. However, in this case the background is a little bit higher.
I have not tested it for IP or WB.