Related topics: Alphabetical list, Cellular marker proteins, Neurotransmission
See list of additional Calbindin antibodies at Linscott’s Directory
On September 23, 2015
Simon Boggild wrote:
I have used this antibody on 10 µm cryosections of mouse embryonic tissue for immunofluorescence. I observed very strong specific staining both in nervous and peripheral tissues with minimal background even though using a concentrated dilution.
On January 19, 2015
Hande Login wrote:
I performed IHC with this antibody on 12 um cryo sections of mouse olfactory bulb sections. I got excellent specific staining of interneurons without any background.
Antibody dilution: 1:500
On August 8, 2014
Maj Ulrichsen wrote:
I have used this antibody on 20 um spinal cord cryo-sections at a dilution of 1:500. It works very well and shows low background staining along with intense cytoplasm and nuclear staining of cells.
On March 29, 2012
Simon Jensen, Aarhus wrote:
DAB staining of tissue with this antibody showed high specificity and very low background at a concentration of 1:2000. For fluorescence staining of tissue. the optimal concentration was 1:1000.
This was also tested on neurons in culture where a clear difference between Calbindin-positive and -negative was seen at a concentration of 1:1000.
This was not tested for IP or WB.